Dear all
Now you get a chance to win $3000 with the Nikon Small World competition! Please also send us your most beautiful images so we will put them on our gallery! 
Please submit by April 30, 2025 via this link.
Useful tips on the Nikon E-learning platform
Dear all
Have a quick look at Nikon’s e-learning platform! There are many useful tips and reminders of what we teach during the LCI training and course. 
For example, here is a reminder of how refractive index mismatch distorts your images and when you should be concerned.
Invitation to submit nominations for the Lennart Nilsson Award 2025
Karolinska Institutet hereby invites nominations for the Lennart Nilsson Award 2025.
The Lennart Nilsson foundation was established in 1998 in order to bestow an award in recognition of the world-renowned Swedish photographer Lennart Nilsson and his extraordinary body of work. Its main aim is to promote education, training and research within the medical, biological and engineering sciences through the use of images.
This is achieved through the Lennart Nilsson Award, an international award bestowed annually upon an individual in recognition of outstanding contributions within the realm of scientific photography.
The nominees should fulfil the following criteria:
- Work in the spirit of Lennart Nilsson
- Make the invisible visible
- Drive the development of imaging
- Reveal sciences to the world in beautiful, unique and powerful ways
- Visualize a scientific breakthrough
- Image reality in a surprising way
Candidates should be active mainly in the Life Sciences and use pictorial representation as an explanatory medium. Users of animation technology are also eligible. The independence of the nominee should be clearly described in the nomination letter.
The nomination form should be filled out and sent in together with a motivation letter, articles, technical descriptions, and pictorial material. Up to three letters with references and comments from experts may be included. Please attach the material as one PDF-file if possible and send to fonder@ki.se no later than Wednesday April 16, 2025.
The awarded amount is SEK 120 000 and the prize ceremony will take place in connection with the installation ceremony for new professors at Karolinska Institutet, Stockholm, Sweden.
More information about the Award can be found on http://ki.se/en/about/the-lennart-nilsson-award
For further questions please contact fonder@ki.se
High Speed Multiphoton Imaging of Dynamic Processes In Vivo at Stockholm University
Intravital people might be interested in this event at the Intravital Microscopy Stockholm University facility:
Clearing live samples!
A couple of super cool papers about transiently clearing live samples to obtain better images!
On Bioarxiv
- In Vivo Optical Clearing of Mammalian Brain, Franzesi et al
- Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo, Inagaki et al
In Science
The online LCI microscopy course: Microscopy: improve your imaging skills – from sample preparation to image analysis.
Dear all
It is my pleasure to invite you to follow, online, free of charge and without registration, the LCI facility microscopy course Microscopy: improve your imaging skills – from sample preparation to image analysis. The public program starts on Tuesday next week (28th of Jan) and runs until the 13th of February.
As usual, all lectures are broadcasted live on Zoom and posted on Youtube every day, for the benefit of our far-away colleagues.
The aim for this course is to improve the microscopy skills of students and researchers who have already used a microscope to acquire digital images of fluorescent samples but feel that more knowledge could help them.
Here is a selection of what we will talk about:
- Optics and image formation,
- How microscopes work
- Objectives and refraction index
- Cameras and detectors, Noise and background, Bit depth and saturation
- Sample preparation, Immunostaining, Clearing
- Resolution and contrast, Nyquist sampling, how to choose microscope settings
- Data handling, how to make article figures, Requirements for image analysis, Colocalization, Ethics
- Image processing and analysis
Check our course webpage to see the course schedule (Broadcasted activities are in blue) and the Zoom link. Here is a full description of the course.
Scroll down to read the kind testimonies of our dear students!
We hope that you will enjoy the LCI facility online microscopy course!
Kindly forward to anyone who might be interested.
The LCI team
Correlative microscopy webinar
For those of you who are interested in Correlative microscopy where one overlays the data acquired from different imaging modalities, Zeiss organises a webinar about combining light microscopy, EM microscopy and Z ray data for very large samples.
Here is the link.
Direct label of primary antibodies with the FlexAble kit
Some of our users have tested the ProteinTech FlexAble kit to directly label primary antibody and they are very happy so we thought we should share!
It only takes 10min to label the primary. It saves time for the sample staining procedure and it saves cute animals when one stops ordering secondary antibodies! In the hand of our users, the images were as bright or brighter than the same labelling with primary + secondary.
And you can ask for a free sample to test! :):):)
Registrations to the LCI microscopy course 2025 will soon close!
There are a few spots left at our course (27 Jan – 14 Feb 2025): ‘Microscopy: improve your imaging skills – from sample preparation to image analysis’ (6 credits). Application deadline: 5th of November 2024.
This course is completely unique in that it is a highly hands-on course but because it runs completely remotely, your hands will be on your own microscope and your own sample.
- To see how the course can help your microscopy project, check out the course schedule 2025 and the alumni testimonies on the course webpage.
- You can read the course syllabus and apply on this page. Make sure you check the eligibility criteria and click on the + sign at the top for more information.
- If you cannot apply to the course, you can anyway follow any of the lectures (in blue on the course schedule) as they will be publicly broadcasted live on Zoom and accessible to anyone without registration. The schedule and zoom link are available on the course page.
The purpose of the LCI facility microscopy course is to provide PhD students, researchers and core facility staff who have some prior experience of microscopy with enough theoretical and practical knowledge about their OWN sample and their OWN microscope, to enable them to:
- assess and improve their sample so that it becomes suitable for data extraction from fluorescence images,
- make best use of the hardware available in their lab/facility,
- fully understand the acquisition parameters they need to set in their own microscope software,
- design their experiment from scientific question to image analysis using a strong knowledge base.
The aim is to provide you with tools to acquire on ANY wide field, confocal or light sheet microscope, images of your samples that reliably answer your scientific question.
The course is free of charge. Contact us (LiveCellImaging@ki.se) for enquiries.
Gisele is hiring!
At the LCI, we have a long standing collaboration with Gisele Miranda, a great and kind image analyst affiliated to BIIF. Gisele is now starting her own image analysis lab! She is currently hiring 2 PhD students so if you know anyone interested, please spread the word. Here is what Gisele says:
I am excited to share that I have recently started my own research group as a SciLifeLab Fellow at KTH Royal Institute of Technology and the Science for Life Laboratory in Stockholm, Sweden. My group will focus on developing and applying cutting-edge Machine Learning techniques to deepen our understanding of cellular behavior and interactions. With a strong emphasis on generative AI, we aim to explore diverse data types, such as microscopy images and omics data, to simulate and predict biological behavior. Our work will focus on mapping complex relationships between single-cell and spatial features, driving new insights into the underlying architecture of cellular networks.
As part of building my group, I currently have two open PhD positions. I would deeply appreciate your help in spreading the word about these opportunities to potential candidates who are interested in working at the intersection of AI and biology.
https://www.kth.se/lediga-jobb/751352?l=en
https://www.kth.se/lediga-jobb/758900?l=en
Thank you for your support, and I look forward to staying connected as my group takes shape!
Best regards,
Gisele